rme2s (Cell Signaling Technology Inc)
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Rme2s, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rme2s/pm25893041__ml500467h_si_001-110-14-15
Average 86 stars, based on 1 article reviews
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Lysis:Article Title: Type I and II PRMTs inversely regulate post-transcriptional intron detention through Sm and CHTOP methylation Article Snippet: .. Lysis for western blotting was accomplished as above and the antibodies used included Article Title: Type I PRMTs and PRMT5 Inversely Regulate Post-Transcriptional Intron Detention Article Snippet: The next morning, Protein G agarose (Millipore-Sigma 16-201) was equilibrated in lysis buffer and added to the lysates at 4 °C with gentle rotation. .. The beads were washed three times with lysis buffer followed by resuspension in 1x Laemmli buffer for western blotting with CHTOP (as above), SNRPB (as above), Article Title: Type I and II PRMTs inversely regulate post-transcriptional intron detention through Sm and CHTOP methylation Article Snippet: The next morning, Protein G agarose (Millipore-Sigma) was equilibrated in lysis buffer and added to the lysates at 4°C with gentle rotation. .. The beads were washed three times with lysis buffer followed by resuspension in 1× Laemmli buffer for western blotting with CHTOP (as above), SNRPB (as above), Western Blot:Article Title: Type I and II PRMTs inversely regulate post-transcriptional intron detention through Sm and CHTOP methylation Article Snippet: .. Lysis for western blotting was accomplished as above and the antibodies used included Article Title: Type I PRMTs and PRMT5 Inversely Regulate Post-Transcriptional Intron Detention Article Snippet: The next morning, Protein G agarose (Millipore-Sigma 16-201) was equilibrated in lysis buffer and added to the lysates at 4 °C with gentle rotation. .. The beads were washed three times with lysis buffer followed by resuspension in 1x Laemmli buffer for western blotting with CHTOP (as above), SNRPB (as above), Article Title: Independent transcriptomic and proteomic regulation by type I and II protein arginine methyltransferases Article Snippet: .. Antibodies used in the western blot include: FUS and TAF15 (see above), Rme1 (CST 8015S), Article Title: Type I and II PRMTs inversely regulate post-transcriptional intron detention through Sm and CHTOP methylation Article Snippet: The next morning, Protein G agarose (Millipore-Sigma) was equilibrated in lysis buffer and added to the lysates at 4°C with gentle rotation. .. The beads were washed three times with lysis buffer followed by resuspension in 1× Laemmli buffer for western blotting with CHTOP (as above), SNRPB (as above), Article Title: Type I and II PRMTs inversely regulate post-transcriptional intron detention through Sm and CHTOP methylation Article Snippet: .. This was followed by addition of Laemmli buffer to 1× and western blotting with H3 (Abcam, ab1791), GAPDH (Abcam, ab9484), U1-70k (Santa Cruz Biotechnology, sc-390988), LaminB2 (Thermo Fisher Scientific, MA1-06104), CHTOP (LSBio, LS-B11259-50), Rme2a (CST, 13522), SNRPB (ProteinTech, 16807-1-AP), SNRPD3 (Abcam, ab157118), and SDS Page:Article Title: Article Snippet: .. Lysates were resolved on SDS PAGE, transferred to the PVDF membrane and immunoblotted with Membrane:Article Title: Article Snippet: .. Lysates were resolved on SDS PAGE, transferred to the PVDF membrane and immunoblotted with |

