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rme2s  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc rme2s
    Rme2s, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rme2s/pm25893041__ml500467h_si_001-110-14-15
    Average 86 stars, based on 1 article reviews
    rme2s - by Bioz Stars, 2026-10
    86/100 stars

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    Related Articles

    Lysis:

    Article Title: Type I and II PRMTs inversely regulate post-transcriptional intron detention through Sm and CHTOP methylation
    Article Snippet: .. Lysis for western blotting was accomplished as above and the antibodies used included Rme2s (CST, 13222), Rme2a (CST, 13522S), GAPDH (Abcam, ab9484), PRMT1 (Millipore, 07-404), PRMT5 (Millipore, 07-405), and FLAG (Sigma-Aldrich, F1804). .. A549 cells were grown with 0.01% DMSO, 1 μM GSK591 (Cayman), or 1 μM MS023 (Cayman) for 2 days.

    Article Title: Type I PRMTs and PRMT5 Inversely Regulate Post-Transcriptional Intron Detention
    Article Snippet: The next morning, Protein G agarose (Millipore-Sigma 16-201) was equilibrated in lysis buffer and added to the lysates at 4 °C with gentle rotation. .. The beads were washed three times with lysis buffer followed by resuspension in 1x Laemmli buffer for western blotting with CHTOP (as above), SNRPB (as above), Rme2s (CST, 13222), and Rme2a (CST, 13522) antibodies. .. The dCas9-KRAB-MeCP2 expression vector (VB900120-5303pyt) was purchased from VectorBuilder (Santa Clara, CA).

    Article Title: Type I and II PRMTs inversely regulate post-transcriptional intron detention through Sm and CHTOP methylation
    Article Snippet: The next morning, Protein G agarose (Millipore-Sigma) was equilibrated in lysis buffer and added to the lysates at 4°C with gentle rotation. .. The beads were washed three times with lysis buffer followed by resuspension in 1× Laemmli buffer for western blotting with CHTOP (as above), SNRPB (as above), Rme2s (CST, 13222), and Rme2a (CST, 13522) antibodies. .. The dCas9-KRAB-MeCP2 expression vector (VB900120-5303pyt) was purchased from VectorBuilder (Santa Clara, CA).

    Western Blot:

    Article Title: Type I and II PRMTs inversely regulate post-transcriptional intron detention through Sm and CHTOP methylation
    Article Snippet: .. Lysis for western blotting was accomplished as above and the antibodies used included Rme2s (CST, 13222), Rme2a (CST, 13522S), GAPDH (Abcam, ab9484), PRMT1 (Millipore, 07-404), PRMT5 (Millipore, 07-405), and FLAG (Sigma-Aldrich, F1804). .. A549 cells were grown with 0.01% DMSO, 1 μM GSK591 (Cayman), or 1 μM MS023 (Cayman) for 2 days.

    Article Title: Type I PRMTs and PRMT5 Inversely Regulate Post-Transcriptional Intron Detention
    Article Snippet: The next morning, Protein G agarose (Millipore-Sigma 16-201) was equilibrated in lysis buffer and added to the lysates at 4 °C with gentle rotation. .. The beads were washed three times with lysis buffer followed by resuspension in 1x Laemmli buffer for western blotting with CHTOP (as above), SNRPB (as above), Rme2s (CST, 13222), and Rme2a (CST, 13522) antibodies. .. The dCas9-KRAB-MeCP2 expression vector (VB900120-5303pyt) was purchased from VectorBuilder (Santa Clara, CA).

    Article Title: Independent transcriptomic and proteomic regulation by type I and II protein arginine methyltransferases
    Article Snippet: .. Antibodies used in the western blot include: FUS and TAF15 (see above), Rme1 (CST 8015S), Rme2s (CST 13222S), Rme2a (CST 13522S); Secondary antibody was TidyBlot-HRP (Bio-Rad STAR209P). ..

    Article Title: Type I and II PRMTs inversely regulate post-transcriptional intron detention through Sm and CHTOP methylation
    Article Snippet: The next morning, Protein G agarose (Millipore-Sigma) was equilibrated in lysis buffer and added to the lysates at 4°C with gentle rotation. .. The beads were washed three times with lysis buffer followed by resuspension in 1× Laemmli buffer for western blotting with CHTOP (as above), SNRPB (as above), Rme2s (CST, 13222), and Rme2a (CST, 13522) antibodies. .. The dCas9-KRAB-MeCP2 expression vector (VB900120-5303pyt) was purchased from VectorBuilder (Santa Clara, CA).

    Article Title: Type I and II PRMTs inversely regulate post-transcriptional intron detention through Sm and CHTOP methylation
    Article Snippet: .. This was followed by addition of Laemmli buffer to 1× and western blotting with H3 (Abcam, ab1791), GAPDH (Abcam, ab9484), U1-70k (Santa Cruz Biotechnology, sc-390988), LaminB2 (Thermo Fisher Scientific, MA1-06104), CHTOP (LSBio, LS-B11259-50), Rme2a (CST, 13522), SNRPB (ProteinTech, 16807-1-AP), SNRPD3 (Abcam, ab157118), and Rme2s (CST, 13222) antibodies. ..

    SDS Page:

    Article Title:
    Article Snippet: .. Lysates were resolved on SDS PAGE, transferred to the PVDF membrane and immunoblotted with Rme2s (Cell Signalling Technology 13222), Y12 (Abcam 3138) SmD1 (Abcam 50940), SmD3 (Abcam 121129), Actin (Abcam 3280), rabbit anti-‐H3R2me2s (Millipore), rabbit anti-‐ H4R3me2s (Abcam), mouse anti-‐H3 (Abcam), mouse anti-‐H4 (Abcam). .. Signal detection was achieved with IRDye680RD Goat anti-‐Mouse IgG (LI-‐COR Biosciences) and IRDye800CW Goat anti-‐Rabbit IgG (LI-‐COR Biosciences) on the Odyssey scanner (LI‐COR Biosciences).

    Membrane:

    Article Title:
    Article Snippet: .. Lysates were resolved on SDS PAGE, transferred to the PVDF membrane and immunoblotted with Rme2s (Cell Signalling Technology 13222), Y12 (Abcam 3138) SmD1 (Abcam 50940), SmD3 (Abcam 121129), Actin (Abcam 3280), rabbit anti-‐H3R2me2s (Millipore), rabbit anti-‐ H4R3me2s (Abcam), mouse anti-‐H3 (Abcam), mouse anti-‐H4 (Abcam). .. Signal detection was achieved with IRDye680RD Goat anti-‐Mouse IgG (LI-‐COR Biosciences) and IRDye800CW Goat anti-‐Rabbit IgG (LI-‐COR Biosciences) on the Odyssey scanner (LI‐COR Biosciences).



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    (A) Schematic representation of the GFP-Trap-based IP-mass spectrometry (MS) approach. (B) List of GFP-RMET interacting proteins identified by IP-MS. HEK293T cells were transfected with GFP-RMET, and half the cells were treated with AdOx for 3 days and subjected to IP-MS analysis. The number of peptides identified for each protein is listed. (C) Far-western analysis of the interaction between a GST-SART3(HAT) and RMET. HEK293T cells were transfected with GFP-EV (empty vector) or the GFP-RMET fusion. After a 3-day treatment with AdOx, total lysates were probed with GST-SART3(HAT) and detected with anti-GST antibody. (D) GST-SART3(HAT) pull-downs using biotinylated <t>fibrillarin</t> GAR peptides. GST-TDRD3(Tudor) and GST-SMN(Tudor) are controls. A mild buffer (150 mM NaCl) was used. (E) GST-SART3(HAT) pull-downs using biotinylated coilin GAR peptides and using mild buffer (300 mM NaCl) and RIPA. Experiments (C–E) were independently repeated three times, with similar results.
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    Image Search Results


    (A) Schematic representation of the GFP-Trap-based IP-mass spectrometry (MS) approach. (B) List of GFP-RMET interacting proteins identified by IP-MS. HEK293T cells were transfected with GFP-RMET, and half the cells were treated with AdOx for 3 days and subjected to IP-MS analysis. The number of peptides identified for each protein is listed. (C) Far-western analysis of the interaction between a GST-SART3(HAT) and RMET. HEK293T cells were transfected with GFP-EV (empty vector) or the GFP-RMET fusion. After a 3-day treatment with AdOx, total lysates were probed with GST-SART3(HAT) and detected with anti-GST antibody. (D) GST-SART3(HAT) pull-downs using biotinylated fibrillarin GAR peptides. GST-TDRD3(Tudor) and GST-SMN(Tudor) are controls. A mild buffer (150 mM NaCl) was used. (E) GST-SART3(HAT) pull-downs using biotinylated coilin GAR peptides and using mild buffer (300 mM NaCl) and RIPA. Experiments (C–E) were independently repeated three times, with similar results.

    Journal: Cell reports

    Article Title: SART3 reads methylarginine-marked glycine- and arginine-rich motifs

    doi: 10.1016/j.celrep.2024.114459

    Figure Lengend Snippet: (A) Schematic representation of the GFP-Trap-based IP-mass spectrometry (MS) approach. (B) List of GFP-RMET interacting proteins identified by IP-MS. HEK293T cells were transfected with GFP-RMET, and half the cells were treated with AdOx for 3 days and subjected to IP-MS analysis. The number of peptides identified for each protein is listed. (C) Far-western analysis of the interaction between a GST-SART3(HAT) and RMET. HEK293T cells were transfected with GFP-EV (empty vector) or the GFP-RMET fusion. After a 3-day treatment with AdOx, total lysates were probed with GST-SART3(HAT) and detected with anti-GST antibody. (D) GST-SART3(HAT) pull-downs using biotinylated fibrillarin GAR peptides. GST-TDRD3(Tudor) and GST-SMN(Tudor) are controls. A mild buffer (150 mM NaCl) was used. (E) GST-SART3(HAT) pull-downs using biotinylated coilin GAR peptides and using mild buffer (300 mM NaCl) and RIPA. Experiments (C–E) were independently repeated three times, with similar results.

    Article Snippet: Fibrillarin peptide Rme2s (SDMA) GG[Rme2s]G[Rme2s]GGGF[Rme2s] G[Rme2s]G[Rme2s]GGGG-Biotin , CPC Scientific , N/A.

    Techniques: Mass Spectrometry, Transfection, Western Blot, Plasmid Preparation

    (A) Western blot analysis of SART3 expression in CRISPR-mediated SART3-knockout (KO) HeLa cells and the stable restoration with GFP fusions to WT and mutant (Y112A and W377A) SART3. (B) IP/western blot analysis of the SART3 interaction with coilin and fibrillarin. Restored HeLa SART3-KO cells in (A) were used. (C) IP/western blot validation of the interaction between SART3 and a subset of spliceosome proteins identified by IP-MS. Restored HeLa SART3-KO cells in (A) were used. (D) Sashimi plots depicting exon skipping in the representative genes TMEM255B and KIAA0895 in HeLa SART3-KO cells restored with WT (red) and mutant (purple) SART3. False discovery rate < 0.05. (E) Semiquantitative RT-PCR supporting the TMEM255B and KIAA0895 exon skipping analysis using HeLa control cells (endogenous SART3), SART3-KO control cells (no SART3), and HeLa sgSART3-KO cells restored with WT GFP-SART3 or the Y112A and W377A mutants (restored), as shown in (A). Three biological repeats were performed ( n = 3). The PCR products were quantified by densitometric analysis, and the exon skipping ratio is shown in a percentage expressed as the mean ± SEM. Unpaired t test was used to calculate p values: ** p < 0.01 and *** p < 0.001.

    Journal: Cell reports

    Article Title: SART3 reads methylarginine-marked glycine- and arginine-rich motifs

    doi: 10.1016/j.celrep.2024.114459

    Figure Lengend Snippet: (A) Western blot analysis of SART3 expression in CRISPR-mediated SART3-knockout (KO) HeLa cells and the stable restoration with GFP fusions to WT and mutant (Y112A and W377A) SART3. (B) IP/western blot analysis of the SART3 interaction with coilin and fibrillarin. Restored HeLa SART3-KO cells in (A) were used. (C) IP/western blot validation of the interaction between SART3 and a subset of spliceosome proteins identified by IP-MS. Restored HeLa SART3-KO cells in (A) were used. (D) Sashimi plots depicting exon skipping in the representative genes TMEM255B and KIAA0895 in HeLa SART3-KO cells restored with WT (red) and mutant (purple) SART3. False discovery rate < 0.05. (E) Semiquantitative RT-PCR supporting the TMEM255B and KIAA0895 exon skipping analysis using HeLa control cells (endogenous SART3), SART3-KO control cells (no SART3), and HeLa sgSART3-KO cells restored with WT GFP-SART3 or the Y112A and W377A mutants (restored), as shown in (A). Three biological repeats were performed ( n = 3). The PCR products were quantified by densitometric analysis, and the exon skipping ratio is shown in a percentage expressed as the mean ± SEM. Unpaired t test was used to calculate p values: ** p < 0.01 and *** p < 0.001.

    Article Snippet: Fibrillarin peptide Rme2s (SDMA) GG[Rme2s]G[Rme2s]GGGF[Rme2s] G[Rme2s]G[Rme2s]GGGG-Biotin , CPC Scientific , N/A.

    Techniques: Western Blot, Expressing, CRISPR, Knock-Out, Mutagenesis, Reverse Transcription Polymerase Chain Reaction, Control

    Journal: Cell reports

    Article Title: SART3 reads methylarginine-marked glycine- and arginine-rich motifs

    doi: 10.1016/j.celrep.2024.114459

    Figure Lengend Snippet:

    Article Snippet: Fibrillarin peptide Rme2s (SDMA) GG[Rme2s]G[Rme2s]GGGF[Rme2s] G[Rme2s]G[Rme2s]GGGG-Biotin , CPC Scientific , N/A.

    Techniques: Virus, Recombinant, Protease Inhibitor, Membrane, Mutagenesis, SYBR Green Assay, Knock-Out, Plasmid Preparation, Software

    Journal: eLife

    Article Title: Type I and II PRMTs inversely regulate post-transcriptional intron detention through Sm and CHTOP methylation

    doi: 10.7554/eLife.72867

    Figure Lengend Snippet:

    Article Snippet: Antibody , Anti-Rme2s (Rabbit polyclonal) , CST , Cat#: 13222SRRID: AB_2714013 , WB: 1:2000.

    Techniques: Transfection, Construct, Mutagenesis, Plasmid Preparation, Software